Fast optically sectioned fluorescence HiLo endomicroscopy
نویسندگان
چکیده
منابع مشابه
Fast optically sectioned fluorescence HiLo endomicroscopy.
We describe a nonscanning, fiber bundle endomicroscope that performs optically sectioned fluorescence imaging with fast frame rates and real-time processing. Our sectioning technique is based on HiLo imaging, wherein two widefield images are acquired under uniform and structured illumination and numerically processed to reject out-of-focus background. This work is an improvement upon an earlier...
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We present an endomicroscope apparatus that exhibits out-of-focus background rejection based on wide-field illumination through a flexible imaging fiber bundle. Our technique, called HiLo microscopy, involves acquiring two images, one with grid-pattern illumination and another with standard uniform illumination. An evaluation of the image contrast with grid-pattern illumination provides an opti...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2012
ISSN: 1083-3668
DOI: 10.1117/1.jbo.17.2.021105